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目的建立山楂叶提取物固体自乳化颗粒中牡荆素鼠李糖苷含量的测定方法。方法采用Diamonsil-C18色谱柱(200 mm×4.6 mm,5μm);四氢呋喃-甲醇-乙腈-2.8%冰醋酸(114:9:9:748)为流动相,检测波长为330 nm,流速为0.8 mL/min。结果牡荆素鼠李糖苷在19.8～198.0μg/mL范围内线性关系良好(r=0.9994),平均回收率为100.98%,RSD为1.21%。结论该方法简便、准确、重复性好,为山楂叶提取物固体自乳化颗粒的质量控制提供参考。
Objective To establish a method for the determination of rhamnoside in the self-emulsifying granules of hawthorn leaf extract. Methods Diamonsil-C18 column (200 mm × 4.6 mm, 5 μm) and tetrahydrofuran-methanol-acetonitrile-2.8% glacial acetic acid (114: 9: 9: 748) were used as the mobile phase. The detection wavelength was 330 nm and the flow rate was 0.8 mL / min. Results The results showed that the rhamnoside in the range of 19.8-198.0 μg / mL showed a good linearity (r = 0.9994) with an average recovery of 100.98% and a RSD of 1.21%. Conclusion The method is simple, accurate and reproducible. It provides a reference for the quality control of self-emulsifying granules of hawthorn leaf extract.