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为探讨Ｐ５３基因点突变与子宫颈癌发生发展的关系。采用聚合酶链反应及其单链构象多态性技术，对６１例子宫颈癌基因组ＤＮＡ的Ｐ５３基因点突变进行检测，分析基因表达与临床病理的关系。结果：聚合酶链反应单链构象多形态性分析位点位于Ｐ５３基因外显子５～８区段，在整个基因组ＤＮＡ中有５例发生Ｐ５３点突变，突变率为８．２％，其中４例Ｐ５３点突变位于外显子７，且均为ＨＰＶ阴性病例；另一例点突变位于外显子５并有ＨＰＶ１８感染。结果提示：ＨＰＶ感染在大多数宫颈癌中发生，Ｐ５３基因外显子５～８的突变，主要在ＨＰＶ阴性的宫颈癌中发生，也偶可发生于ＨＰＶ阳性宫颈癌，宫颈癌发生与人乳头瘤病毒感染及Ｐ５３基因功能失活有关。
To investigate the relationship between the point mutation of P53 and the occurrence and development of cervical cancer. Polymerase chain reaction and its single strand conformation polymorphism technique were used to detect the point mutation of P53 gene in 61 cases of cervical cancer genomic DNA and to analyze the relationship between gene expression and clinical pathology. Results: Polymorphism analysis of single strand conformation polymorphism in the polymerase chain reaction (PCR) locus was located in the exon 5 to 8 of P53 gene. P53 point mutation occurred in 5 of the whole genomic DNA with a mutation rate of 8.2%, of which 4 The case of P53 point mutation located in exon 7, and are HPV-negative cases; another case of point mutations in exon 5 and HPV18 infection. The results suggest that HPV infection occurs in most cervical cancers. The mutation of exon 5 to 8 of P53 gene mainly occurs in HPV-negative cervical cancer, and may even occur in HPV-positive cervical cancer, cervical cancer and human papilla Tumor virus infection and P53 gene function inactivation.