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目的:探讨甲醛对小鼠外周血免疫球蛋白(IgG、IgM)、细胞因子白细胞介素-4(IL-4)合成水平的影响。方法:80只成年昆明种小鼠随机分成对照组及甲醛低、中、高剂量组,对照组除不给甲醛外,其他处理同实验组,低、中、高剂量组每天2h吸入不同浓度气态甲醛(0、2.5、10及30mg/m3),持续2周,采用ELISA法测定各组小鼠外周血清中IgG、IgM及IL-4水平。结果:外周血中IgG、IgM的表达随甲醛浓度的升高而增加,高剂量组明显高于对照组和低剂量组,差异有统计学意义(P<0.05);IL-4的水平亦随甲醛暴露浓度的升高而升高,中、高剂量组均显著高于对照组,差异有统计学意义(P<0.05)。结论:甲醛吸入使小鼠体内IgG、IgM和IL-4水平升高,并有诱导变态反应和哮喘发生的可能。
Objective: To investigate the effect of formaldehyde on the levels of peripheral blood immunoglobulin (IgM) and interleukin-4 (IL-4) in mice. Methods: Eighty adult Kunming mice were randomly divided into control group and low, middle and high dose formaldehyde groups. The control group was given the same experimental group as the experimental group except for the control group. The low, medium and high dose groups were inhaled with different concentrations of gaseous phase Formaldehyde (0, 2.5, 10 and 30mg / m3) for 2 weeks. The levels of IgG, IgM and IL-4 in peripheral blood of the mice in each group were measured by ELISA. Results: The expression of IgG and IgM in peripheral blood increased with the increase of formaldehyde concentration, while the high dose group was significantly higher than the control group and the low dose group (P <0.05). The level of IL-4 also increased with the increase of formaldehyde concentration Formaldehyde exposure concentration increased and increased, medium and high dose groups were significantly higher than the control group, the difference was statistically significant (P <0.05). Conclusion: Inhalation of formaldehyde can increase the levels of IgG, IgM and IL-4 in mice and induce allergic reaction and asthma.