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目的检测肺癌和癌旁正常组织中Gab2蛋白及其mRNA的表达情况,探讨Gab2与肺癌发生发展的关系。方法采用PV-9003免疫组织化学法检测75例肺癌组织和25例癌旁正常组织中Gab2蛋白表达水平,实时荧光定量RT-PCR方法检测15对肺癌组织和相对应的癌旁正常肺组织中Gab2基因mRNA表达状况。结果 Gab2蛋白在30例鳞癌、30例腺癌和15例其他类型癌中的阳性表达率分别为66.67%,46.67%和80%,高于癌旁正常肺组织(P<0.01);15例肺癌组织中Gab2 mRNA表达高于相对应的癌旁正常肺组织(P<0.05)。结论 Gab2在肺癌的发生发展中可能起重要作用,其表达增强可能诱导了肺癌的发生。
Objective To detect the expression of Gab2 protein and its mRNA in lung cancer and adjacent normal tissues, and to explore the relationship between Gab2 and the occurrence and development of lung cancer. Methods The expression of Gab2 protein in 75 lung cancer tissues and 25 paracancerous normal tissues were detected by PV-9003 immunohistochemical method. The real-time fluorescence quantitative RT-PCR method was used to detect the expression of Gab2 in 15 paired lung cancer tissues and corresponding adjacent normal lung tissues. Gene mRNA expression status. Results The positive expression rates of Gab2 protein in 30 squamous cell carcinomas, 30 adenocarcinomas and 15 other types of carcinomas were 66.67%, 46.67% and 80%, respectively, which were higher than those in normal lung tissues (P<0.01). The expression of Gab2 mRNA in lung cancer tissues was higher than that in corresponding normal lung tissues (P<0.05). Conclusion Gab2 may play an important role in the development of lung cancer. The enhanced expression of Gab2 may induce the occurrence of lung cancer.