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目的通过用两种洗涤溶液制备冰冻解冻去甘油红细胞结果进行质量分析,来比较应用不同洗涤溶液,一类是9%氯化钠、羟乙基淀粉130氯化钠注射液、0.9%氯化钠;一类是9%氯化钠、0.9%氯化钠分别进行解冻红细胞洗涤,选择合适制备冰冻解冻去甘油红细胞的洗涤方法。方法随机抽取2010年采血6d之内新鲜全血32份,通过手工制备冰冻红细胞:6d之内(2～6℃保存)新鲜全血经过25min,2300转,离心后去除血浆,将血液倒入三珠空袋内,用无菌接驳机连接输血器,应用甘油160ml,25min加入振荡器60次/min,室温沉淀30min后放入-80℃冰箱速冻,1个月后进行解冻。随机分为A、B两组,每组16袋,应用ACP215血液处理仪进行解冻红细胞。A组洗涤液包括9%氯化钠、羟乙基淀粉130氯化钠、0.9%氯化钠这三种溶液;B组洗涤液包括9%氯化钠、0.9%氯化钠这两种溶液。用两种不同洗涤溶液进行冰冻解冻去甘油红细胞洗涤,通过检测项目为红细胞回收率%,白细胞残留率%,血小板残留率%,甘油含量,血浆游离血红蛋白含量,体外溶血等指标进行检测分析。结果 A组解冻红细胞:红细胞回收率81.0%±2.6%,白细胞残留率0.67%±0.14%,血小板残留率0.00%,甘油含量4.8±0.43g/L,血浆游离血红蛋白含量0.62±0.11g/L,体外溶血13%±5%。B组解冻红细胞:红细胞回收率82.0%±2.3%,白细胞残留率0.61%±0.26%,血小板残留率0.42%±0.1%,甘油含量(5.1±0.13)g/L,血浆游离血红蛋白含量(0.53±0.12)g/L,体外溶血12%±4%。两组结果均符合国家标准,且无统计学意义。结论通过手工制备冰冻红细胞后,应用ACP215血液处理仪制备冰冻解冻去甘油红细胞分别使用A、B两种洗涤液,根据统计学分析洗涤效果无统计学意义,检测结果均达到国家标准,可以确定在洗涤中不加入羟乙基淀粉130氯化钠注射液,检测结果也能达到国家标准。
OBJECTIVE To compare the results of different freeze-thaw deoxyglucose red blood cells by using two kinds of washing solution to compare the different washing solutions, one is 9% sodium chloride, 130 mg sodium hydroxyethyl starch injection, 0.9% sodium chloride ; One is 9% sodium chloride, 0.9% sodium chloride were thawed red blood cells were washed, select the appropriate preparation of frozen thawing to glycerol red blood cell washing method. Methods 32 fresh blood samples were collected randomly within 6 days after blood collection in 2010. Frozen red blood cells were prepared by hand: Fresh whole blood within 6 days (stored at 2 ~ 6 ℃) was centrifuged at 2500rpm for 2300rpm. After centrifugation, plasma was removed and the blood was poured into tris Pearls empty pouch, connected with sterile transfusion device blood transfusion, glycerol 160ml, 25min by adding shaker 60 times / min, precipitated at room temperature for 30min and then placed in -80 ℃ freezer, thawed after 1 month. Randomly divided into A, B two groups, each group of 16 bags, the application of ACP215 hematology thawing red blood cells. A group of washing solution, including 9% sodium chloride, 130 hydroxyethyl starch, sodium chloride, 0.9% sodium chloride solution of these three; Group B washing solution, including 9% sodium chloride, 0.9% sodium chloride solution . Two kinds of washing solutions were used to freeze and thaw the glycerol red blood cells. The detection items were red blood cell recovery rate, leukocyte residual rate, residual platelet percentage, glycerol content, plasma free hemoglobin content and hemolysis in vitro. Results A group of thawed erythrocytes: erythrocyte recovery rate of 81.0% ± 2.6%, leucocyte residual rate of 0.67% ± 0.14%, platelet residual rate of 0.00%, glycerol content of 4.8 ± 0.43g / L, plasma free hemoglobin content of 0.62 ± 0.11g / L, In vitro hemolysis 13% ± 5%. Group B thawed erythrocytes: erythrocyte recovery 82.0% ± 2.3%, leucocyte residual rate 0.61% ± 0.26%, platelet residual rate 0.42% ± 0.1%, glycerol content 5.1 ± 0.13 g / L, plasma free hemoglobin content 0.53 ± 0.12) g / L, hemolysis in vitro 12% ± 4%. The two groups of results are in line with national standards, and no statistical significance. Conclusion After preparation of frozen erythrocytes by hand, the ACP215 hematology apparatus was used to prepare frozen thawed glycerol red blood cells. A and B washing solutions were respectively used. According to the statistical analysis, the washing effect was not statistically significant, and the test results reached the national standard, Wash does not add hydroxyethyl starch 130 sodium chloride injection, the test results can reach the national standard.