采用细胞病变结晶紫染色法测定具有特异诊断依据的16例狂犬病患者血清中TNF活性。结果显示患者组TNF活性指数明显高于对照组（P＜0．01）.表明狂犬病毒感染除其特异性地侵犯神经组织，造成神经功能严重紊乱外，也可通过TNF这一类与组织的炎症及坏死密切相关的细胞因子，造成系统性损害，加重病情，加速狂犬病患者的死亡。用细胞病变结晶紫染色法测定狂犬病患者血清中TNF活性，结果判断客观，容易推广。 The serum levels of TNF in 16 rabies patients with specific diagnosis were determined by crystal violet staining of cytopathic effect. The results showed that the TNF activity index of patients was significantly higher than the control group (P <0.01), indicating that rabies virus infection in addition to its specific violations of nerve tissue, resulting in serious neurological disorders, but also through the TNF and this type of tissue Inflammation and necrosis of closely related cytokines, causing systemic damage, aggravate the condition, accelerate the death of rabies patients. Cystine violet staining with cytopathic effect in rabies patients serum TNF activity, the results of objective judgment, easy to promote.