论文部分内容阅读
目的：研究哮喘豚鼠肺组织Ｇ蛋白α亚基含量的表达变化及胸腺素对其的影响。方法：用卵蛋白雾化吸入致敏的方法复制豚鼠哮喘模型，并用胸腺素治疗，Ｗｅｓｔｅｒｎ ｂｌｏｔ杂交分析法测定Ｇα含量。结果：哮喘组豚鼠肺组织Ｇｉα（１２６％ ±１１％ ）、Ｇｑα（１２２％ ±１２％ ）较正常对照组显著升高（Ｐ均＜ ０．０５）；而胸腺素组Ｇｉα、Ｇｑα（分别为９７％ ±９％ 和９８％ ±１１％ ）较哮喘组显著降低（Ｐ均＜ ０．０５）；胸腺素组Ｇｓα（１０７％ ±６％ 和１０４％ ±５％ ）无显著变化（Ｐ均＞ ０．０５）。结论：哮喘肺组织Ｇｉα、Ｇｑα表达增加在哮喘发病中可能占有一定地位，而胸腺素通过降低Ｇｉα、Ｇｑα在哮喘豚鼠肺组织的表达变化达到了治疗或缓解哮喘的目的。
Objective: To study the expression of G protein α subunit in lung tissue of asthmatic guinea pig and the effect of thymosin on it. Methods: Guinea pig asthma model was replicated by inhalation sensitization with egg protein and treated with thymosin. Gα content was determined by Western blot. Results: The levels of Giα (126% ± 11%) and Gqα (122% ± 12%) in asthmatic guinea pigs were significantly higher than those in normal controls (P <0.05) 97% ± 9% and 98% ± 11%) were significantly lower than those in asthma group (P <0.05). There was no significant change in Gsα (107% ± 6% and 104% ± 5% 0.05). Conclusion: The increased expression of Giα and Gqα in asthmatic lung tissue may play a certain role in the pathogenesis of asthma. Thymosin achieves the goal of treating or relieving asthma by reducing the expression of Giα and Gqα in the lung tissue of asthmatic guinea pigs.