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目的检测PCA3在前列腺癌(PCa)患者前列腺按摩后尿液中的表达情况,并讨论其临床意义。方法从56例PCa患者、23例良性前列腺增生(BPH)患者和9例健康男性志愿者(对照组)前列腺按摩后初始尿液中分离细胞,采用RT-PCR方法检测其中PCA3的表达情况。结果BPH和对照组未见PCA3阳性表达,而PCa患者PCA3阳性率为81.3%(39/48),两者差异具有统计学意义(P<0.01)。结论前列腺按摩后尿液中PCA3的检测有望成为早期诊断PCa的一种较敏感的方法,也有望成为PCa治疗后监测的一种方法。
Objective To detect the expression of PCA3 in the urine of patients with prostate cancer (PCa) after prostate massage and to discuss its clinical significance. Methods PCA3 was detected by RT-PCR in 56 cases of PCa, 23 cases of benign prostatic hyperplasia (BPH) and 9 healthy male volunteers (control group) after prostatic massage. Results No positive expression of PCA3 was found in BPH and control groups, while the positive rate of PCA3 in PCa was 81.3% (39/48). There was significant difference between the two groups (P <0.01). Conclusions The detection of PCA3 in urine after prostate massage is expected to be a sensitive method for early diagnosis of PCa. It is also expected to become a monitoring method after PCa treatment.