本文介绍从亚急性硬化性全脑炎(SSPE)病人的脑组织匀浆中分离提取IgG作为免疫试剂。作者选择经临床、血液学和病理学三方面证实的SSPE患者的脑组织用0.05 M磷酸缓冲液(内加0.45％氯化钠,0.02％叠氮钠和0.05M6-氨基己酸,pH 7.2简称PBSAA),制成10％(重量/体积)的匀浆。使用10万g离心1小时,然后再悬浮在PBSAA This article describes the isolation and extraction of IgG as an immunological reagent from brain tissue homogenates from patients with sub-acute sclerosing full-brain disease (SSPE). The authors selected brain tissue from patients with SSPE who were clinically, hematologically, and pathologically proven to be challenged with 0.05 M phosphate buffer (plus 0.45% sodium chloride, 0.02% sodium azide and 0.05M 6-aminocaproic acid, pH 7.2 for short PBSAA) to make a 10% (w / v) homogenate. Centrifuge for 1 hour at 100,000 g and resuspend in PBSAA