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利用105条S系列和100条Operon随机引物,对小麦-簇毛麦代换系(6V／6A)、两个易位系(6VS／6AL,6VS／6DL)及亲本簇毛麦(VV)、硬粒小麦(AABB)、栽培小麦(AABBDD)的多态性进行了筛选分析。80.95％的S系列引物扩增出了结果,且条带较清晰;在100条OP系列引物中均扩增出结果,条带清晰可见。从205个随机引物中发现,只有1个引物OPW03在含有簇毛麦V染色体的4个材料中均扩增出1条约570 bp的谱带,而在栽培小麦和硬粒小麦中没有发现。因此,可以推测这个分子标记(OPW03-570)是位于簇毛麦V染色体短臂上的。
A total of 105 S series and 100 Operon random primers were used to analyze the genetic diversity of wheat (Triticum aestivum L.) cultivars (6V / 6A), two translocation lines (6VS / 6AL and 6VS / 6DL) Triticum aestivum (AABB), cultivated wheat (AABBDD) polymorphism were screened. 80.95% of the S series primers amplified the results, and the bands are clearer; the results are amplified in 100 OP series primers and the bands are clearly visible. From 205 random primers, only one primer, OPW03, amplified a band of about 570 bp in four materials containing V. napus V chromosomes, but not found in cultivated wheat and durum wheat. Therefore, it can be speculated that this molecular marker (OPW03-570) is located on the short arm of V chromosome in Hamster.