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脂类过氧化作为自由基损伤机体组织细胞的一般机制引起众多学者的极大兴趣,许多研究表明:肿瘤、感染、动脉硬化、梗塞、风湿、肝损伤等多种疾病与自由基损伤有关。脂类过氧化物作为自由基代谢产物,在一定程度上反映着机体的自由基水平。我们基于脂类过氧化物(LPO)在酸性环境加热分解产生丙二醛(MDA),而丙二醛能与硫代巴比妥酸(TBA)发生颜色反应的原理,定量测定样品中LPO和MDA的总含量,我们称其为TBA反应物(TBA-R)。
Lipid peroxidation as a general mechanism of free radical damage to tissue cells aroused great interest of many scholars, many studies have shown that: tumor, infection, arteriosclerosis, infarction, rheumatism, liver damage and other diseases and free radical damage. Lipid peroxide as a free radical metabolite, to some extent, reflects the body’s free radical levels. Based on the principle that lipid peroxides (LPO) are heated and decomposed in acidic environment to produce malondialdehyde (MDA), and MDA can react with thiobarbituric acid (TBA) quantitatively, the effects of LPO and The total content of MDA, we call it TBA reactant (TBA-R).