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目的 :探讨醋柳总黄酮 (TFH)及其单体降压及心血管综合保护作用机制。方法 :紫外分光光度法和放射免疫法检测TFH、槲皮素 (Que)、异鼠李素 (Isor)对培养的兔主动脉平滑肌细胞 (ASMC)及兔血浆血管紧张素转换酶 (ACE)活性及血管紧张素 (Ang )生成的影响 ,用卡托普利 (Cap)作对照。结果 :TFH、Que、Isor对 ASMC ACE活性及 Ang 生成有显著持久的抑制作用 ,对血浆 ACE活性及 Ang 生成也有短暂抑制作用 ;TFH、Que、Isor对 ASMC、ACE活性和 Ang 生成抑制作用明显强于兔血浆 ,持续时间较血浆长。结论 :TFH及其单体对 ACE活性及 Ang 生成的抑制作用 ,特别是细胞内作用 ,可能是其降压及心血管综合保护作用的重要机制之一。
Objective: To investigate the mechanisms of antihypertensive and cardiovascular comprehensive protection of total flavonoids from Tissu (Williin) Hort.f. (TFH) and its monomers. Methods: Ultraviolet spectrophotometry and radioimmunoassay were used to detect the activity of TFH, quercetin (Que) and Isor in rabbit aortic smooth muscle cells (ASMC) and rabbit plasma angiotensin-converting enzyme (ACE) activity And angiotensin (Ang) production, using Captopril (Cap) as a control. RESULTS: TFH, Que, and Isor had a significant and sustained inhibitory effect on ACE activity and angiogenesis, as well as short-term inhibition of plasma ACE activity and angiogenesis. TFH, Que and Isor had a significantly stronger inhibitory effect on ACE activity and Ang production In rabbit plasma, the duration is longer than plasma. CONCLUSION: The inhibitory effect of TFH and its monomers on ACE activity and angiogenesis, especially intracellular effects, may be one of the important mechanisms of antihypertensive and cardiovascular protection.