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目的:通过对7月龄与12月龄雌雄大鼠去势致骨质疏松模型的比较,为建立男性及老年性骨质疏松模型提供实验数据。方法:取7月龄、12月龄雌雄SD大鼠分别随机分为伪手术组与去势组,手术15周后检测动物的体重、骨生物力学、组织计量学、血清生化、骨指数等指标,进行组间比较。结果:7、12月龄雌雄大鼠去势后胫骨近端的骨松质骨量减少,股骨、胫骨力学性能有不同程度的下降,股骨骨长度、重量等有不同程度的减少。7月龄雌性大鼠去势后体重增长加快、碱性磷酸酶(ALP)增加,雄性大鼠去势后体重增长减缓、ALP减少。与相应的7月龄组相比,12月龄组胫骨近端的骨松质骨量减少,股骨、胫骨力学性能未见明显改变。与雌性动物相比,雄性动物的股骨骨重量、长度等明显增加,骨力学性能增强,左侧胫骨近端骨松质骨量减少。结论:7、12月龄雌雄大鼠去势后15周均可发生骨质疏松症,而7月龄大鼠去势造成的骨质疏松更显著。与7月龄大鼠相比,12月龄雌雄大鼠可形成增龄性骨质疏松,而雌性动物随着增龄造成的骨质疏松更为明显。
OBJECTIVE: To provide experimental data for the establishment of male and senile osteoporosis models by comparing the castrated osteoporosis models in male and female rats of 7 months and 12 months. Methods: Male and female SD rats aged 7 months and 12 months were randomly divided into sham operation group and castrated group. After 15 weeks of operation, the body weight, bone biomechanics, histomorphometry, serum biochemistry, bone index and other indexes , For comparison between groups. Results: At 7 and 12 months old, the amount of cancellous bone in the proximal tibia was decreased in females and rats, the mechanical properties of the femur and tibia decreased in varying degrees, and the length and weight of the femur decreased in varying degrees. Seven-month-old female rats weight gain after exacerbation, alkaline phosphatase (ALP) increased, weight loss after castration slowed the male rats, ALP decreased. Compared with the corresponding 7-month-old group, the bone mass in the proximal tibia of 12-month-old group decreased and the mechanical properties of the femur and tibia did not change significantly. Compared with females, the weight and length of the femur in males increased significantly, the bony mechanical properties increased, and the amount of bone cancellous bone in the proximal tibia decreased. CONCLUSION: Osteoporosis can occur in both male and female rats at 7 and 12 months of age after 15 weeks of castration, whereas osteoporosis caused by castration in 7-month-old rats is more pronounced. Compared with 7-month-old rats, 12-month-old male and female rats can form age-related osteoporosis, while osteoporosis in females is more pronounced with age.