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目的观察复方中药对大鼠坐骨神经损伤后神经组织中生长相关蛋白-43(GAP-43)表达的影响。方法 SD大鼠156只,随机分为假手术组、对照组与中药组,后两组切断右侧坐骨神经并缝合。中药组给予复方神肌再生冲剂200mg.kg-1.d-1溶于2ml0.9%NaCl溶液中灌胃,对照组给予0.9%Nacl溶液2ml灌胃,假手术组仅显露右侧坐骨神经,术后不作处理。分别于术后1、3、7、14、21及28天取吻合口远端的神经,采用免疫组化和实时荧光定量PCR方法检测损伤神经组织中GAP-43的表达水平。结果实验组GAP-43蛋白阳性染色光密度值(OD)在术后7、14和21天明显高于对照组(P<0.05),术后3、7和14天,实验组坐骨神经中GAP-43 mRNA的表达高于对照组(P<0.05)。结论复方神肌再生冲剂可增加大鼠坐骨神经损伤后神经组织中GAP-43的表达,从而有利于神经再生。
Objective To observe the effects of compound Chinese herbs on the expression of GAP-43 in nerve tissue after sciatic nerve injury in rats. Methods One hundred and sixty-five SD rats were randomly divided into sham operation group, control group and TCM group. The latter two groups were cut off the right sciatic nerve and sutured. The Chinese medicine group was given 200mg.kg-1.d-1 of Compound Shenpi Regenerative Granules in 2ml0.9% NaCl solution, while the control group was given 2ml of 0.9% Nacl solution. The sham operation group only showed the right sciatic nerve After no treatment. The nerves at distal part of anastomosis were taken at 1, 3, 7, 14, 21 and 28 days after operation. The expression of GAP-43 in injured nerve tissue was detected by immunohistochemistry and real-time fluorescence quantitative PCR. Results The positive staining OD value of GAP-43 protein in experimental group was significantly higher than that in control group at 7, 14 and 21 days after operation (P <0.05). At 3, 7 and 14 days after operation, GAP- 43 mRNA expression was higher than the control group (P <0.05). Conclusion Compound Shenjin Recipe can increase the expression of GAP-43 in nerve tissue after sciatic nerve injury in rats, which is beneficial to nerve regeneration.