论文部分内容阅读
目的:探讨不同磷脂补充对小鼠心肌线粒体功能的影响。方法:雄性昆明种小鼠48只,随机分为4组:安静对照组、运动对照组、大豆磷脂补充组和肝磷脂补充组。后3组每天早、晚两次灌胃,肝磷脂补充组为18mg/ml的肝磷脂悬浊液,大豆磷脂补充组为18mg/ml的大豆磷脂悬浊液,运动对照组灌胃生理盐水,共2周。饲养2周后除安静对照组外,其余各组均进行一次力竭游泳。运动后即刻分别测定小鼠心肌线粒体NO、游离Ca2+和ATP含量。结果:(1)力竭运动后运动对照组NO含量显著高于安静对照组,磷脂补充组显著低于运动对照组;3个运动组力竭运动后Ca2+和ATP含量均显著低于安静对照组,两个磷脂补充组Ca2+和ATP含量显著高于运动对照组。两磷脂补充组各指标均无明显差异。(2)两个磷脂补充组小鼠游泳力竭时间均显著长于运动对照组,且肝磷脂补充组小鼠力竭游泳时间略长于大豆磷脂补充组,但无统计学意义。结论:磷脂补充可以削弱力竭运动对线粒体的影响,使ATP生成增多,运动时间延长。两种磷脂补充效果无明显不同。
Objective: To investigate the effects of different phospholipids supplementation on myocardial mitochondrial function in mice. Methods: Forty-eight male Kunming mice were randomly divided into 4 groups: control group, exercise control group, soybean phospholipid supplement group and heparin supplementation group. The latter three groups were given gavage twice daily by morning and evening. The heparin group was supplemented with 18mg / ml heparin, the soybean phospholipid supplemented group was 18mg / ml soybean phospholipid suspension. The exercise group was given normal saline, A total of 2 weeks. After 2 weeks of feeding, except the quiet control group, the rest of the groups were exhausted swimming. Immediately after exercise, myocardial mitochondrial NO, free Ca2 + and ATP were measured respectively. Results: (1) The content of NO in exercise control group after exhaustive exercise was significantly higher than that in quiet control group, the phospholipid supplement group was significantly lower than that in exercise control group. The content of Ca2 + and ATP in exhaustive exercise of three exercise groups were significantly lower than that in quiet control group , Ca2 + and ATP contents in two phospholipid supplement groups were significantly higher than that in exercise control group. Two phospholipid supplement group of each index no significant difference. (2) The swimming time of mice in two phospholipid supplement groups was significantly longer than that in the control group, and the exhaustive swimming time in the heparin group was slightly longer than that in soybean phospholipid supplement group, but there was no statistical significance. Conclusion: Supplementing with phospholipids can weaken the effect of exhaustive exercise on mitochondria, and increase ATP production and exercise time. Two kinds of phospholipid supplement effect no significant difference.