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目的 分析广东省严重急性呼吸综合征 (SARS)病例SARS CoV抗体检出情况。方法 分别对 2 92份广东省SARS临床确诊病例、39份疑似病例和 30 3份健康体检人员 (一般人群 )血清标本 ,采用酶联免疫方法进行SARS冠状病毒IgG抗体检测。结果 SARS临床确诊病例的SARS CoVIgG抗体阳性率为 5 8 90 % (1 72 / 2 92 ) ,高于疑似病例及一般人群抗体阳性率 (分别为 1 8 0 0 %、2 31 % )(P <0 0 1 )。分析 2 92份临床确诊病例血清标本 ,结果显示有明确接触史者抗体阳性率 (72 6 7% )高于无明确接触史者 (4 1 98% ) (P <0 0 1 )。不同性别抗体阳性率差异无显著性 ,不同年龄组抗体阳性率差异有显著性 ,4 0～ 5 9岁年龄组最高 (P <0 0 1 )。流行早期病例抗体阳性率 (6 7 4 3% )高于后期(31 94 % )。SARS冠状病毒IgG抗体在发病 1 0d后能较可靠检出且能维持较长时间。结论 SARS冠状病毒IgG抗体检测结果与临床诊断存在一定差异 ,IgG抗体检测是有效的辅助诊断手段 ,但不能用于早期诊断。实验室血清学检测结果进一步证明接触史是SARS病例诊断的重要依据之一。
Objective To analyze the detection of SARS CoV antibody in cases of severe acute respiratory syndrome (SARS) in Guangdong Province. Methods Serological samples of 2 92 SARS cases diagnosed in Guangdong, 39 suspected cases and 30 3 healthy people (general population) were detected by enzyme-linked immunosorbent assay (ELISA). Results The positive rate of SARS CoVIgG antibody in the clinically confirmed SARS cases was 58 890% (1 72/2 92), which was higher than that of the suspected cases and the general population (180%, 231% respectively) (P < 0 0 1). Analysis of 2 92 serum samples from clinically confirmed cases showed that the positive rate of antibodies with a clear history of exposure (72 6 7%) was higher than that without clear exposure history (41 98%) (P 0 01). There was no significant difference in the positive rate of antibody between different sexes. The positive rate of antibody in different age groups was significantly different (P 0 01). The positive rate of early epidemic cases was higher than that of the late stage (694.3%). SARS coronavirus IgG antibody in the onset of 10d can be more reliably detected and can maintain a long time. Conclusion The results of SARS coronavirus IgG antibody test and clinical diagnosis are different. IgG antibody test is an effective diagnostic aid, but it can not be used in early diagnosis. Laboratory serological test results further prove that contact history is an important basis for the diagnosis of SARS cases.