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目的：揭示细胞凋亡敏感性在人癌细胞体外恶性转化动态过程中的变化。方法：用已获部分恶 性的ＳＶ４０Ｔ转染的人支气管上皮细胞系Ｍ为材料，通过凋亡ＴＤＴ原位标记、染色体ＦＩＳＨ、ＲＮＡ及蛋白检测等技 术，研究同一株细胞恶性转化过程中凋亡及ｂｃｌ－２、Ｐ５３基因的变化。结果：恶性转化的Ｍ后代细胞较未转化的 Ｍ前代细胞对顺铂诱发细胞凋亡现象不敏感；ｂｃｌ－２基因的转录和表达在Ｍ后代细胞明显高于Ｍ前代细胞，而 且在皿细胞恶性转化进程中呈积累现象；Ｐ５３蛋白在Ｍ前、后代细胞均表达。结论：ｂｃｌ－２过表达使细胞对凋亡 敏感性下降在人支气管上皮细胞恶性转化中起重要作用；Ｐ５３蛋白的灭活不是此ＳＶ４０Ｔ转染细胞恶性转化进程 中ｂｃｌ－２表达积累的主要原因。
OBJECTIVE: To reveal the changes of apoptosis sensitivity in the dynamic process of malignant transformation of human cancer cells in vitro. METHODS: Human bronchial epithelial cell line M transfected with partially malignant SV40T was used to study apoptosis during the malignant transformation of the same cell by techniques such as apoptosis TDT in situ labeling, chromosomal FISH, RNA and protein detection. Changes in bcl-2 and P53 genes. RESULTS: Malignant transformed M progeny cells were not sensitive to cisplatin-induced cell apoptosis compared to untransformed M progenitor cells; bcl-2 gene transcription and expression was significantly higher in M offspring cells than in M progenitor cells, and Accumulation was observed during the malignant transformation of cells; P53 protein was expressed in both pre- and post-M cells. Conclusion: The over-expression of bcl-2 may decrease the apoptosis sensitivity of cells in the malignant transformation of human bronchial epithelial cells. The inactivation of P53 protein is not the main reason for the accumulation of bcl-2 in the malignant transformation process of SV40T transfected cells. .