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目的：建立人端粒酶催化亚基蛋白质 Ｒ Ｎ Ａ（ｈ Ｔ Ｒ Ｔ Ｒ Ｎ Ａ）逆转录聚合酶链反应（ Ｒ Ｔ Ｐ Ｃ Ｒ），进行恶性肿瘤细胞和胃癌组织检测，探讨其在癌变中的作用，提供一种癌症检测的新方法。方法：制备癌细胞株和胃癌组织端粒酶提取液和总 Ｒ Ｎ Ａ，运用端粒重复扩增法（ Ｔ Ｒ Ａ Ｐ）和 ｈ Ｔ Ｒ Ｔ Ｒ Ｎ Ａ Ｒ Ｔ Ｐ Ｃ Ｒ 扩增法检测癌细胞株和胃癌组织端粒酶活性和ｈ Ｔ Ｒ Ｔ Ｒ Ｎ Ａ 表达。结果：８ 株癌细胞和 ６ 例胃癌组织均有端粒酶活性和 ｈ Ｔ Ｒ Ｔ Ｒ Ｎ Ａ 表达；９ 例正常组织、１ 例早期胃癌胃体粘膜和 ５例胃炎标本均无端粒酶活性和ｈ Ｔ Ｒ Ｔ 表达。结论：端粒酶ｈ Ｔ Ｒ Ｔ ｍ Ｒ Ｎ Ａ 的 Ｒ Ｔ Ｐ Ｃ Ｒ 检测方法同端粒酶活性测定 Ｔ Ｒ Ａ Ｐ法有良好的一致性，有可能成为利用端粒酶标志检测癌症的一种更为有效的检测方法。
Objective: To establish a human telomerase catalytic subunit protein R N A (h T R T R N A) reverse transcriptase polymerase chain reaction (R T P C R) for the detection of malignant tumor cells and gastric cancer, and to investigate The role of cancer in providing a new method of cancer detection. Methods: The telomerase extract and total RN A were prepared from cancer cell lines and gastric cancer tissues. The telomere repeat amplification method (TRPA) and hTRTRNAPR-RPR amplification method were used. Telomerase activity and hTRTRNA expression were detected in cancer cell lines and gastric cancer tissues. Results: The telomerase activity and hTRTRNA expression were detected in 8 cancer cells and 6 gastric cancer tissues. There were no telomerase activity in 9 cases of normal tissues, 1 case of early gastric mucosa and 5 cases of gastritis. h T R T expression. Conclusion: The R T P C R detection method of telomerase h T R T m R N A is in good agreement with the telomerase activity assay T R A P method, and may be used to detect cancer using the telomerase enzyme marker. A more effective detection method.