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目的 :研究I期乳腺癌p53基因的蛋白表达水平及其与年龄、肿瘤大小、病理分型、分类、分级、浸润程度、预后等临床、病理指标的关系。方法 :应用 p53单克隆抗体 ,免疫组化S -P法 ,回顾性检测112例I期乳腺癌组织石蜡切片标本的 p53蛋白表达水平 ,比较其与各病理指标的关系。结果 :1.p53蛋白在I期乳腺癌的阳性表达率为60.71% (68/112)。2.p53蛋白阳性表达率与组织学分级呈正相关 ,且有统计学意义 ,(P<0.05)。3.p53蛋白阳性表达率与淋巴结转移率有正相关的趋势 ,但无统计学意义 (P>0.05)。4.p53蛋白阳性表达率与5a、10a、15a健在率呈负相关趋势 ,但无统计学差异 (P>0.05)。5.p53蛋白表达与ER、PR、年龄、病理组织学类型无明显相关性。结论 :p53蛋白表达是乳腺癌恶性程度及预后估计中有一定价值的参考指标 ,但就其与临床、病理、预后等各参数的内在联系尚有待进一步研究。
Objective: To study the protein expression of p53 gene in stage I breast cancer and its relationship with age, tumor size, pathological classification, classification, grading, infiltration, prognosis and other clinical and pathological parameters. Methods: The expression of p53 protein in 112 cases of stage I breast cancer tissue paraffin sections was retrospectively detected by p53 monoclonal antibody and immunohistochemical S-P method. The relationship between the expression of p53 protein and the pathological parameters was analyzed. The positive rate of p53 protein in stage I breast cancer was 60.71% (68/112). The positive rate of p53 protein was positively correlated with histological grade (P <0.05). There was a positive correlation between the positive rate of p53 protein and lymph node metastasis, but there was no statistical significance (P> 0.05). There was a negative correlation between the positive rate of p53 protein and 5a, 10a, 15a, but no significant difference (P> 0.05). 5.p53 protein expression and ER, PR, age, histopathological type no significant correlation. Conclusions: The expression of p53 protein is a valuable index for evaluating the malignancy and prognosis of breast cancer. However, its intrinsic relationship with clinical, pathological and prognostic parameters remains to be further studied.