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【目的】初步了解广州市社区食物中毒事件中诺如病毒的分子流行病学情况。【方法】在2003年10月至12月期间,从5个不同流行现场,采集急性期胃肠炎患者的粪便标本76份,用RT-PCR对标本中的诺如病毒核酸进行扩增,将阳性产物回收、纯化并测序,与GenBank中的相关序列进行比较分析,绘制系统发生树。【结果】76份样品中共检出阳性36份,阳性率为47.37%。从5个流行现场获得5个代表株,分别为DX1106、PY1106、HM1121、PY1202和HD1219,其中DX1106与PY1202的序列完全一致,而其他毒株间的序列同源性在35%～85%之间。系统发生树分析发现5个代表株都属于GⅡ组诺如病毒,但分属于不同的基因型。其中DX1106与PY1202株属于GⅡ-3型,HM1121和HD1219株属于GⅡ-4型,PY1106株介于GⅡ-6、7、8型之间,尚不能确定型别。【结论】广州市存在诺如病毒引起的食物中毒,流行优势株为GⅡ组,但其基因型别存在多样性。
【Objective】 To understand the molecular epidemiology of norovirus in food poisoning in Guangzhou. 【Method】 From October to December 2003, 76 stool specimens from patients with acute gastroenteritis were collected from 5 different epidemic sites. The norovirus nucleotides in samples were amplified by RT-PCR. The positive product was recovered, purified and sequenced, compared with the related sequences in GenBank, and the phylogenetic trees were drawn. 【Result】 A total of 36 positive samples were detected in 76 samples, the positive rate was 47.37%. Five representative strains were obtained from five endemic sites, which were DX1106, PY1106, HM1121, PY1202 and HD1219, respectively. The sequences of DX1106 and PY1202 were identical, while those of other strains were between 35% and 85% . Phylogenetic tree analysis showed that all five representative strains belonged to GII norovirus, but belonged to different genotypes. Among them, DX1106 and PY1202 belonged to GⅡ-3, HM1121 and HD1219 belonged to GⅡ-4, and PY1106 belonged to GⅡ-6, 7 and 8, which was not yet confirmed. 【Conclusion】 The food poisoning caused by Norovirus in Guangzhou City exists. The predominant strain is GⅡ, but its genotype is diverse.