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目的对不同生长年限及不同部位川明参Chuanmingshen violaceum Sheh et Shan进行红外光谱及二阶导数图谱研究。方法采用溴化钾压片法和傅里叶变换红外光谱法对不同生长年限川明参及川明参根茎和茎叶进行红外光谱及二阶导数图谱进行研究。结果随着生长年限的增长,川明参次生代谢所累积的物质逐渐增多,在3366cm~(-1)、2927cm~(-1)、1634cm~(-1)、1416cm~(-1)、1516cm~(-1)、1080cm~(-1)、1020cm~(-1)和928cm~(-1)等位置出现特征吸收峰,主要化学成为多糖、香豆素、皂苷和甾体等。根茎和茎叶化学成分差异明显。结论采用红外光谱法对不同生长年限及不同部位川明参进行研究,图谱特征明显,方法简便,适合川明参的鉴别与质量评价。
OBJECTIVE To study the infrared spectra and second derivative spectra of Chuanmingshen violaceum Sheh et Shan with different growth years and different parts. Methods Infrared spectra and second derivative spectra of rhizomes and stems and leaves of M. chinensis were determined by potassium bromide tabletting method and Fourier transform infrared spectroscopy. Results With the growth of the growing years, the accumulation of secondary metabolites in Chuanming minggong increased gradually. The results showed that there was a significant increase in the accumulation of secondary metabolites at 3366cm -1, 2927cm -1, 1634cm -1, 1416cm -1, 1516cm ~ (-1), 1080cm ~ (-1), 1020cm ~ (-1) and 928cm ~ (-1). The main chemical groups were polysaccharides, coumarins, saponins and steroids. The chemical compositions of rhizomes and stems and leaves differed significantly. Conclusions Infrared spectroscopy is used to study the different growth stages and the different parts of Chuanmingshen, the characteristics of which are obvious. The method is simple and convenient, which is suitable for the identification and quality evaluation of Chuanming.