目的：将ＨＬＡＤＲＢ１基因ｃＤＮＡ片段反向插入逆转录病毒质粒ＺＩＰｎｅｏＳＶ（×）ＢａｍＨＩ位点中，构建了ＨＬＡＤＲＢ１基因的逆转录病毒反义ＲＮＡ重组表达载体，用脂质体法导入ＰＡ３１７细胞。方法：用免疫磁珠法分离，富集ＣＤ３４＋脐血造血干／祖细胞。含编码人ＨＬＡＤＲＢ１基因的ｐｃＤＶ１质粒，用ＢａｍＨＩ酶解，回收ＨＬＡＤＲＢ１ｃＤＮＡ片段，将ｃＤＮＡ片段反向插入ｐＺＩＰｎｅｏＳＶ（×）逆转录病毒载体，经扩增、抽提、酶切鉴定，用脂质体将反义ＲＮＡ重组体导入到ＰＡ３１７细胞，用含Ｇ４１８３００μｇ／ｍｌ培养液筛选，获抗性克隆，流式细胞仪检测ＨＬＡＤＲ抗原阳性细胞数。结果：重组质粒转染ＰＡ３１７细胞后，其病毒滴度达１×１０５ＣＦＵ／ｍｌ，脐血造血干／祖细胞经免疫磁珠富集后，ＣＤ３４＋细胞高达８５．０％～９０．０％，导入反义ＲＮＡ的脐血干细胞，其ＨＬＡＤＲ抗原表达从导入前的４５．０％降至２８．０％，抑制率达３８２％，而导入空载体后从５６．０％降至４５．０％，差异不显著。结论：ＨＬＡＤＲ的反义ＲＮＡ能导入脐血干细胞，降低ＨＬＡＤＲ抗原的表达 OBJECTIVE: To reversely insert the cDNA fragment of HLADRB1 gene into the ZIPneoSV (×) BamHI site of retroviral plasmid to construct a retroviral antisense RNA recombinant expression vector of HLADRB1 gene and introduce it by liposome PA317 cells. Methods: Immunomagnetic beads were used to separate and enrich CD34 + cord blood hematopoietic stem / progenitor cells. PcDV1 plasmid containing human HLA-DRB1 gene was digested with BamHI, and the HLA-DRB1 cDNA fragment was recovered. The cDNA fragment was reversely inserted into pZIP-neoSV retrovirus vector, amplified, extracted and identified by restriction enzyme digestion. The antisense RNA recombinant was introduced into PA317 cells by liposome, and then screened with G418300μg / ml culture medium to obtain resistant clones. The number of HLADR antigen positive cells was detected by flow cytometry. Results: After the recombinant plasmids were transfected into PA317 cells, their virus titer reached 1 × 105CFU / ml. CD34 + cells were enriched up to 85.0% -90.0% after being enriched by immunomagnetic beads. The antisense RNA of umbilical cord blood stem cells, the HLA DR antigen expression from pre-45.0% to 28.0%, the inhibition rate of 38  2%, but after the introduction of empty vector decreased from 56.0% to 45 .0%, the difference is not significant. Conclusion: HLADR antisense RNA can be introduced into cord blood stem cells and reduce the expression of HLA  DR antigen