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松针褐斑病(Lecanostictaacicola)是我国南方重要病害。在湿地松(Pinuselliottii)抗褐斑病选育研究的基础上对湿地松针叶中的6-磷酸葡萄糖脱氢酶和苯丙氨酸解氨酶与抗病性的关系进行了研究分析。结果表明二年生健康针叶的6-磷酸葡萄糖脱氢酶的活性水平与针叶的抗病性有密切关系。在测定的13个无性系和单株中,4个易感株的酶活性较低,9个抗病无性系中除个别酶活性与易感株相近外,其余无性系的酶活性和比活性都明显比易感株高。苯丙氨酸解氨酶的株间差异相对较小,但在总体水平上,抗病无性系的酶活性和比活性也比易感株略高。用松针褐斑病菌毒素液处理后,无论是抗病针叶还是易感针叶,6-磷酸葡萄糖脱氢酶的活性均呈上升趋势,升幅为0.5—1倍,但苯丙氨酸解氨酶的活性在用毒素处理后无明显变化。
Pine needle brown spot (Lecanostictaacicola) is an important disease in southern China. Based on the studies on resistance to brown spot disease of Pinus fortunei, the relationship between 6-phosphoglucose dehydrogenase and phenylalanine ammonia-lyase and the disease resistance of Pinus elliottii was studied. The results showed that biennial healthy needles of glucosinolate 6-phosphate dehydrogenase activity level and conifers disease resistance are closely related. Among the 13 clones and plants tested, the susceptibility of 4 susceptible strains was lower than that of susceptible ones except for the activity of individual enzymes and susceptible strains in 9 resistant clones Significantly higher than susceptible plants. Phenylalanine ammonia-lyase isolates showed relatively small isolates, but at the general level, the resistance and specific activities of disease-resistant clones were slightly higher than those of susceptible strains. After treatment with P. cuneata, the activity of glucose-6-phosphate dehydrogenase showed an upward trend of 0.5-1-fold, both for resistant leaf needles and susceptible needles, but phenylalanine The activity of the ammonia-lyase did not change significantly after treatment with the toxin.