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用日本血吸虫可溶性虫卵抗原(SEA)免疫BALB/c 小鼠,取其脾细胞与 SP2/0骨髓瘤细胞按 Kennett 方法作细胞融合,用有限稀释法克隆化2～3次后,建立一株分泌特异性抗体的杂交瘤细胞(A6-B6),其培养上清经浓缩后作免疫球蛋白亚类签定为 IgM,杂交瘤诱生的小鼠腹水经 SEA 免疫
BALB / c mice were immunized with soluble Schistosoma japonicum antigen (SEA) and their spleen cells and SP2 / 0 myeloma cells were fused by Kennett method and cloned by limiting dilution method for 2 to 3 times to establish a strain Specific antibody secreting hybridoma cells (A6-B6), the culture supernatant was concentrated for immunoglobulin subclass was identified as IgM, hybridoma-induced mouse ascites by SEA immune