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目的探讨诱捕受体3(DcR3)蛋白和caspase-3在结直肠癌癌变过程中的表达及其与结直肠癌临床病理特征之间的关系。方法采用免疫组织化学SP法检测70例结直肠癌组织,59例结直肠腺瘤(腺瘤伴低度上皮内瘤变37例,腺瘤伴高度上皮内瘤变22例)和10例结直肠正常粘膜组织中DcR3和caspase-3蛋白的表达,分析其与临床病理特征之间的关系。结果①DcR3在结直肠正常粘膜、腺瘤、结直肠癌组织中的阳性表达率分别为0(0/10)、39.0%(23/59)和70%(49/70),而caspase-3阳性表达率分别为100%(10/10)、33.9%(20/59)、28.6%(20/70);②在结直肠癌组织中DcR3和caspase-3的表达与患者性别、年龄、肿瘤部位、组织类型、分化程度等均无相关性(P>0.05),但DcR3的表达与Dukes分期有明显的相关性,Dukes C/D期者,DcR3阳性表达率低(P<0.05);③在结直肠腺瘤组织中caspase-3的表达与腺瘤分化程度的比较差异有统计学意义(P<0.05),而DcR3和caspase-3的表达与患者的年龄、性别、腺瘤部位、大小、数目、表面形态等差异均无统计学意义(P>0.05)。结论①DcR3和caspase-3可能共同参与了结直肠癌的发生发展;②检测DcR3和caspase-3的表达对于结直肠癌的早期诊断可能有一定的价值。
Objective To investigate the expression of decoy receptor 3 (DcR3) protein and caspase-3 in carcinogenesis of colorectal cancer and its relationship with the clinicopathological features of colorectal cancer. Methods Immunohistochemical SP method was used to detect the expression of MMP-9 in 70 cases of colorectal cancer tissues, 59 cases of colorectal adenoma (adenoma with low-grade intraepithelial neoplasia in 37 cases, adenoma with high-grade intraepithelial neoplasia in 22 cases) and 10 cases of colorectal The expression of DcR3 and caspase-3 in normal mucosa tissues was analyzed and their relationship with clinicopathological features was analyzed. Results ① The positive rates of DcR3 in colorectal mucosa, adenoma and colorectal cancer tissues were 0 (0/10), 39.0% (23/59) and 70% (49/70) respectively, while the positive rates of caspase-3 The expression rates of DcR3 and caspase-3 in colorectal cancer tissues were 100% (10/10), 33.9% (20/59) and 28.6% (20/70) respectively. The expression of DcR3 and caspase- (P> 0.05). However, there was a significant correlation between DcR3 expression and Dukes stage, while the positive rate of DcR3 in Dukes C / D stage was lower than that in Dukes C / D stage (P <0.05) The expression of caspase-3 in colorectal adenoma was significantly different from that in adenoma (P <0.05), while the expressions of DcR3 and caspase-3 were not associated with age, gender, adenoma location, size, Number, surface morphology and other differences were not statistically significant (P> 0.05). Conclusion ①DcR3 and caspase-3 may participate in the development of colorectal cancer. ②Detecting the expression of DcR3 and caspase-3 may be valuable in the early diagnosis of colorectal cancer.