人接触苯会引起白血病,尤其是急性成髓细胞白血病。接触低剂量苯的人,外周血淋巴细胞染色体畸变率增加。大、小鼠接触苯后也观察到白血病和其它肿瘤。有证据表明苯本身不与DNA起反应或引起突变,但可被代谢成遗传毒物。在Ames试验中,即使加以代谢活化苯也无致突变性。作者建立了一种新的体外短期筛选法——微筛法。该法仅用一株E.coli WP2s(λ)菌即可检测所有类别的诱变剂。E.coli WP2s(λ)携带有uvrA突变及在trpE基因上的突变,因此不能进行大的加合物的切补修复,并且其生长依 Exposure to benzene can cause leukemia, especially acute myeloid leukemia. People exposed to low doses of benzene have an increased rate of chromosomal aberrations in peripheral blood lymphocytes. Large and mouse leukemia and other tumors were also observed after exposure to benzene. There is evidence that benzene itself does not react with or cause mutations in DNA but can be metabolized to genetic poisons. In the Ames test, there is no mutagenicity even with metabolic activation of benzene. The author established a new short-term in vitro screening method - micro-sieve method. This method uses only one strain of E. coli WP2s (λ) to detect all classes of mutagens. E. coli WP2s (λ) carries the uvrA mutation and mutations in the trpE gene and therefore can not undergo extensive repair of adducts and its growth depends on