论文部分内容阅读
目的探讨盐酸阿比朵尔体外抗汉坦病毒效果。方法采用流式细胞分析法、间接免疫荧光检测病毒抗原评价盐酸阿比朵尔对汉坦病毒感染的作用方式;流式细胞检测法、RT-PCR半定量法对5mg·L-1盐酸阿比朵尔在感染前24h(-24h)、12h(-12h)及感染后0h、2h4个时间点作用后病毒抗原与核酸进行检测。结果感染前给药浓度组及感染后给药浓度组,病毒感染细胞阳性率、荧光强度随浓度增加下降,具有剂量效应,与病毒对照组比较,有统计学意义(P<0.05);5mg·L-1盐酸阿比朵尔在-24h,-12h,0h,2h4个时间点作用后,可明显降低病毒感染阳性率,具有时间效应;-24h、-12h作用后,病毒mRNA表达下降,与病毒对照组比较,有统计学意义(P<0.05)。结论盐酸阿比朵尔体外具有抗汉坦病毒效应,在病毒进入细胞前及细胞后均有抑制作用,不同时间用药以病毒进入细胞前作用更明显,其抗病毒机制有待进一步研究。
Objective To investigate the anti-Hantavirus effect of abidol hydrochloride in vitro. Methods Flow cytometry and indirect immunofluorescence assay were used to evaluate the effect of abidol hydrochloride on Hantavirus infection. Flow cytometry and semi-quantitative RT-PCR were used to evaluate the effect of abamectin hydrochloride (5 mg · L -1) The virus antigens and nucleic acids were detected at 24 hours (-24h), 12h (-12h) and 0h, 2h after infection. Results The positive rate and the fluorescence intensity of virus infected cells decreased with the increase of the concentration and the dose-effect when compared with the virus control group (P <0.05); 5mg · L-1 abiratix hydrochloride could significantly reduce the positive rate of virus infection at the time of -24h, -12h, 0h and 2h, and had a time effect. After -24h and -12h, the mRNA expression of virus decreased, Virus control group, statistically significant (P <0.05). Conclusion Arbidol hydrochloride has anti-Hantavirus effect in vitro, which inhibits the virus before and after entering the cell. The effect of anti-Hantavirus is more obvious before the virus enters the cell at different times. The anti-virus mechanism remains to be further studied.