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目的通过大孔吸附树脂筛选拳参黄酮抗氧化的最佳活性部位。方法以静态吸附率和解吸率为指标,从中筛选出富集拳参黄酮的最佳树脂,通过黄酮液p H、浓度等性能来确定动态吸附和洗脱的条件,并采用DPPH和ABTS+清除实验来筛选拳参黄酮抗氧化最佳活性部位。结果 X-5大孔吸附树脂对拳参黄酮有较好的吸附分离性能,其静态吸附平衡时间为2h,吸附条件为(上样液p H3、流速1 m L·min-1),黄酮浓度为0.4477 mg·m L-1,分别以40%、60%、80%、95%乙醇洗脱,得A~D部位。各洗脱部位均有一定的抗氧化能力,其中C部位(80%乙醇洗脱部位)清除·DPPH与ABTS+的活性最强,是拳参黄酮抗氧化的最佳活性部位。结论拳参黄酮是一种很好的天然抗氧化剂。
Objective To screen the best active site of the antioxidant activity of Forsythia flavone by macroporous adsorption resin. Methods Based on the static adsorption rate and desorption rate, the optimal resin for enrichment of flavonoids from Fistula was collected. The conditions of dynamic adsorption and elution were determined by the properties of p H and concentration of flavonoids. The DPPH and ABTS + To filter the boxing flavonoids antioxidant the best active site. Results X-5 macroporous resin had good adsorption and separation properties for flavonoids of Flos Sophorae. The static adsorption equilibrium time was 2h, and the adsorption conditions were as follows: sample volume p H3, flow rate 1 m L · min-1, flavonoid concentration 0.4477 mg · m L-1, respectively, with 40%, 60%, 80%, 95% ethanol elution, the A ~ D site. Each elution site has a certain antioxidant capacity, of which C site (80% ethanol elution site) scavenging · DPPH and ABTS + the strongest activity, is the best anti-oxidation Fistula genus flavonoids active site. Conclusion Fistula flavonoids is a good natural antioxidants.