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目的:研究高胆固醇作用下兔oddi括约肌(SO)钙依赖性钾通道β1亚基蛋白水平的变化。方法:制备鼠抗兔SO细胞钙依赖性钾通道β1亚基抗血清,进行免疫组化染色,观察高胆固醇对oddi括约肌钙依赖性钾通道β1亚基蛋白表达的影响。结果:免疫组化染色显示,高胆固醇组SO细胞钙依赖性钾通道β1亚基的蛋白表达降低,半定量分析显示,高胆固醇兔oddi括约肌BKca通道β1亚基蛋白表达水平与对照组比较有统计学意义。结论:高胆固醇可下调兔oddi括约肌BKca通道β1亚基蛋白表达的水平,从而影响BKca通道的功能。
AIM: To investigate the changes of β1 subunit protein in calcium-dependent potassium channel of oddi sphincter (SO) in rabbits under hypercholesterolemia. Methods: The antiserum of calcium-dependent potassium channel β1 subunit of rabbit anti-rabbit SO cells was prepared and immunohistochemically stained to observe the effect of hypercholesterol on the expression of calcium-dependent potassium channel β1 subunit protein in oddi sphincter. Results: Immunohistochemical staining showed that the protein expression of β1 subunit of calcium-dependent potassium channel in SO group was decreased in hypercholesterol group. Semiquantitative analysis showed that the expression of β1 subunit protein in BKCa channel of Oddi sphincter in hypercholesterolemic group was statistically higher than that in control group Significance of learning. Conclusion: Cholesterol can down-regulate the expression of β1 subunit protein of BKca channel in rabbit oddi sphincter, and thus affect the function of BKca channel.