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目的 :建立测定人体内血清中交沙霉素 (Jos)浓度的方法。方法 :采用改进的 HPL C法 ,色谱柱为 SUPEL CODiscovery C1 8(2 5 0 m m× 4.6 mm,5 ︼m) ,流动相为甲醇∶磷酸二氢钾缓冲液 (p H2 .5 ) =(6 2∶ 38) (V/ V) ,紫外检测波长为2 32 nm。结果 :Jos在 0 .0 5～ 3.2 0 ︼g/ ml浓度范围内线性关系良好 ,r=0 .9998。高、中、低 3个浓度的日内、日间的 RSD均小于 10 %。结论 :本法成本低、毒性小、操作简单、结果准确、重现性好 ,适用于临床 Jos血药浓度的监测和药物动力学及生物利用度的研究
Objective: To establish a method for determination of Jos concentration in human serum. Methods: A modified HPL C method was used. The column was SUPER CODiscovery C1 8 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol: potassium dihydrogen phosphate buffer (p H2 .5) = (6 2:38) (V / V), UV detection wavelength of 2 32 nm. Results: Jos had a good linearity in the range of 0. 0 5 ~ 3.2 0 ︼g / ml, r = 0 .9998. High, medium and low concentrations of 3 days, day RSD were less than 10%. Conclusion: This method is low cost, low toxicity, simple operation, accurate results and good reproducibility. It is suitable for the monitoring of clinical serum concentration of Jos and its pharmacokinetics and bioavailability