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目的探索缺氧刺激对大鼠骨髓间充质干细胞(rat bone marrow mesenchyme stem cells,rBMSCs)骨向诱导分化过程中骨钙素(oteocalcin,OC)基因表达的影响。方法本研究把rBMSCs骨向诱导后按不同氧浓度及不同作用时间分组,检测不同作用时间的缺氧对rBMSCs骨向诱导分化过程中OC基因表达的影响。结果缺氧培养对rBMSCs骨向诱导分化中OC基因表达的影响在各时间段均有明显抑制作用且有统计学差别(P<0.05)。结论缺氧培养对rBM-SCs骨向诱导分化过程中OC基因表达抑制作用明显,表明2%缺氧对rBMSCs骨向诱导分化有抑制作用,促使骨改建平衡向破骨方向倾斜。
Objective To explore the effect of hypoxia on the expression of osteocalcin (oteocalcin, OC) during osteogenic differentiation of rat bone marrow mesenchyme stem cells (rBMSCs). Methods In the present study, rBMSCs were induced into osteoblasts and divided into groups according to different oxygen concentrations and different duration of action. The effects of hypoxia at different time on the expression of OC gene in osteogenic differentiation of rBMSCs were observed. Results The effect of hypoxia culture on the expression of OC gene in osteogenic differentiation of rBMSCs was significantly inhibited at different time points (P <0.05). Conclusion The hypoxia culture inhibited the expression of OC gene in osteogenic differentiation of rBM-SCs, which indicated that 2% hypoxia could inhibit the osteogenic differentiation of rBMSCs and promote the balance of osteoclast remodeling toward osteoclasts.