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根据间日疟原虫环子孢子蛋白基因设计合成特异性引物一对，采用ＰＣＲ技术，以Ｄｉｇ－１１－ｄＵＴＰ代替部分ｄＴＩＰ直接扩增并标记含间日疟原虫环子孢子蛋白基因中央重复序列的基因片段作为ＤＮＡ探针并用于检测间日疟原虫感染，结果表明：（１）采用ＰＣＲ法直接制备并标记地高辛探针的方法较ＰＣＲ扩增后再标记的方法更为快速、简便和经济；（２）探针只与间日疟患者血样核酸抽提物杂交阳性，而与其亲缘关系相近的三株恶性疟原虫、利什曼原虫、弓形虫及正常人血核酸抽提物杂交阴性，该探针可以检测出相当于１００μｌ感染血样中４４虫／μｌ的水平；（３）将该探针用于深圳地区及湖北随州地区收集的间日疟患者血样的检测，１４７份镜检阳性的血样中，１１３份杂交阳性，与镜检的符合率为７６．８７％，２０份正常人血杂交阴性。
A pair of specific primers was designed and synthesized according to the circumsporozoite protein gene of Plasmodium vivax. PCR was used to directly amplify and label the central repetitive sequence of Plasmodium vivax sporozoite protein gene by replacing part of dTIP with Dig-11-dUTP The results showed that: (1) The method of direct preparation and labeling of digoxigenin probe by PCR method is more rapid, simpler and more convenient than the method of re-labeling after PCR amplification Economic; (2) The probe only hybridized with the nucleic acid extract of blood samples of Plasmodium vivax, while the three strains of Plasmodium falciparum, Leishmania, Toxoplasma gondii and normal human whose nucleic acids were similar to their counterparts were negative for hybridization , Which can detect the level of 44 insects / μl corresponding to 100 μl of the infected blood sample. (3) The probe was used to detect the blood samples of vivax malaria collected in Shenzhen area and Suizhou area of Hubei province, and 147 were positive Of the blood samples, 113 were positive hybridization, and microscopy coincidence rate of 76.87%, 20 normal subjects Blood hybrids negative.