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菊芋是生物能源和生物炼制的新型原料作物,具有和其他作物不同的秸秆组成.为了解菊芋秸秆的生物转化情况,本研究首先比较了NaOH-H_2O_2、瞬间弹射蒸汽爆破(ICSE)及NaOH-H_2O_2和ICSE联用等3种预处理方法,证明对于菊芋秸秆NaOH-H_2O_2预处理法简单高效.进一步研究显示,NaOH-H_2O_2预处理过程中水洗一次即可显著促进酶解和后续发酵.利用分批补料和补加纤维素酶的方式进行高物料浓度条件下预处理菊芋秸秆的分步水解和乙醇发酵,当物料浓度达到30%(m/V)时,水解72 h的葡萄糖和木糖浓度分别可达143.6 g/L和36.2 g/L.利用木糖-葡萄糖共发酵重组酿酒酵母菌株LX03在菊芋秸秆水解液中进行乙醇发酵,发酵72 h乙醇最高浓度达66.2 g/L(8.27%,V/V),且发酵总糖利用率达86.9%.本研究利用菊芋秸秆水解液发酵获得较高的乙醇产量,为进一步利用菊芋秸秆进行高效生物炼制及高浓度纤维素乙醇生产提供了参考.(图3表1参23)
Jerusalem artichoke is a new type of raw material for bioenergy and bio-refining, and has different composition of straw from other crops.In order to understand the bioconversion of Jerusalem artichoke straw, this study first compared the effects of NaOH-H 2 O 2, ICSE and NaOH- H_2O_2 and ICSE combined three pretreatment methods proved that the Jerusalem artichoke straw NaOH-H_2O_2 pretreatment method is simple and efficient further study showed that the NaOH-H_2O_2 pretreatment process can significantly promote the enzymatic hydrolysis and subsequent fermentation. Fed-batch and supplemented with cellulase were used to pretreat the Jerusalem artichoke straws pretreated with ethanol and ethanol at a high feed concentration. When the feed concentration reached 30% (m / V), the glucose and xylose The concentration of which reached 143.6 g / L and 36.2 g / L, respectively.The fermentation of Jerusalem artichoke straws hydrolyzed by xylose-glucose was carried out by ethanol fermentation.The highest concentration of ethanol reached 66.2 g / L (8.27% , V / V), and the total sugar utilization of the fermentation reached 86.9% .This study used Jerusalem artichoke straws hydrolyzate fermentation to obtain higher ethanol yield, in order to further use of Jerusalem artichoke straw for efficient biological refining and high concentrations of cellulose ethanol Production provides a reference. (Reference 23 in FIG. 3 in Table 1)