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本文旨在研究缺氧刺激条件下中缝背核内远位触液神经元Fos蛋白的表达情况。应用低压氧舱仿海拔8000米高空缺氧模型,采用侧脑室注射CB(霍乱毒素B亚单位)标记中缝背核内的远位触液神经元,并用Fos免疫组化和CB/Fos免疫组化双重染色方法显示Fos在中缝背核远位触液神经元内的表达情况。结果表明中缝背核内存在大量的CB标记神经元(31.16±3.36/每张切片)。缺氧实验组中缝背核内Fos阳性神经元数显著增加(40.28±2.17/每张切片,P<0.05),并可见CB/Fos双标神经元(2.00±0.39/每张切片)。对照组中缝背核内Fos阳性神经元数较少(5.55±0.81/每张切片),未见CB/Fos双标神经元。本文结果提示在缺氧刺激条件下,中缝背核内部分远位触液神经元在脑脑脊液之间的信息传递及功能调节中可能发挥一定作用。
The aim of this study was to investigate the expression of Fos protein in distal synaptic fluid neurons in the dorsal raphe nucleus under hypoxic conditions. The hypothalamic neurons in the dorsal raphe nucleus were labeled with intracerebroventricular injection of CB (cholera toxin B subunit) using hypoxia chamber model at 8000 m altitude hypoxia. Immunohistochemistry with Fos and CB / Fos Double staining showed Fos expression in the dorsal raphe fluid neurons. The results showed that a large number of CB-labeled neurons (31.16 ± 3.36 per slice) were present in the dorsal raphe. The numbers of Fos positive neurons in dorsal raphe nucleus of hypoxia group were significantly increased (40.28 ± 2.17 per slice, P <0.05), and CB / Fos double labeled neurons (2.00 ± 0.39 per slice) were observed. In the control group, the number of Fos-positive neurons in the dorsal raphe nucleus was less (5.55 ± 0.81 per slice), and no CB / Fos double-labeled neurons were found. Our results suggest that under hypoxic conditions, some of the distal dorsal palmpatee neurons in the dorsal raphe may play a role in the information transmission and functional regulation between cerebrum and cerebrospinal fluid.