目的　探讨二硫化碳 (CS2 )对邻菲罗啉化学发光体系中羟自由基 (·OH)产生的影响。方法　应用邻菲罗啉化学发光体系观察不同浓度CS2 的发光动力学曲线、硫脲对发光强度的抑制作用及无CuSO4 和维生素C时CS2 对该体系的影响。结果　随CS2 浓度 (0、2 0、40、80、12 0、16 0mg/ml)的增高 ,发光动力曲线峰值增大 (r=0 .95 5 ,R2 =0 .913,P =0 .0 0 3) ,峰时提前 (r=- 0 .92 7,R2 =0 .86 0 ,P=0 .0 0 8) ,有剂量 -效应关系。硫脲对CS2 诱导的发光强度均有抑制作用 ,但抑制率随CS2 浓度的增高而减小 (r=- 0 .816 ,R2 =0 .6 6 6 ,P =0 .0 48)。当体系中无CuSO4 和维生素C存在时 ,CS2 仍能使发光峰值增高。结论　CS2 在邻菲罗啉化学发光体系中能促进·OH产生和发光峰时提前 Objective To investigate the effect of carbon disulfide (CS2) on the production of hydroxyl radical (· OH) in o-phenanthroline chemiluminescence system. Methods The phenanthroline chemiluminescence system was used to observe the luminescence kinetics curves of different concentrations of CS2, the inhibitory effect of thiourea on the luminescence intensity and the effect of CS2 on the system without CuSO4 and vitamin C. Results With the increase of concentration of CS2 (0,2 0,40,80,12 0,16 0mg / ml), the peak of luminescence kinetic curve increased (r = 0.955, R2 = 0.913, P = 0 .0 0 3). The peak time was earlier (r = - 0.92 7, R2 = 0.86 0, P = 0.08). There was a dose-response relationship. Thiourea inhibited the luminescence intensity induced by CS2, but the inhibition rate decreased with the increase of CS2 concentration (r = -0.816, R2 = 0.666, P = 0.048). When the system does not exist in the presence of CuSO4 and vitamin C, CS2 can still make the peak emission increased. Conclusion CS2 can promote the generation of · OH and the emission peak in the orthophenanthroline chemiluminescence system in advance