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目的:阐明免疫缺陷动物筛选人转移癌组织过程中主要的非正常死亡原因,为人类肿瘤动物模型建立和筛选提供借鉴。方法:采用皮下、原位、肾包膜下移植等多种方法对267只BALB/c裸小鼠接种(或注射)人肾细胞癌、前列腺癌临床标本、DU-145细胞系,分析成瘤率、肝脏病变情况(临床表现、病理切片、死亡时间分布)等。结果:移植肾细胞癌标本的总成瘤率为21.7%(35/161),转移率为1.2%(2/161)。移植前列腺癌临床标本均未成瘤或发生转移;移植DU-145细胞系成瘤率为100%(20/20),转移率为25%(5/20)。肾细胞癌临床标本移植小鼠肝脏病变率为58.4%(94/161),前列腺癌临床标本移植小鼠肝脏病变率为43.4%(46/106),DU-145细胞系移植小鼠无1例肝脏病变发生。移植肾细胞癌、前列腺癌标本肝脏病变小鼠死亡高峰与小鼠总体死亡高峰趋势一致,均发生在每年的冬春季。结论:肝脏病变是造成动物非正常死亡的主要原因,严格控制SPF环境方可保证肿瘤动物模型的顺利建立。
OBJECTIVE: To elucidate the major causes of abnormal death in immunodeficient animal screening of human metastatic carcinomas and provide references for the establishment and screening of human tumor animal models. Methods: 267 BALB / c nude mice were inoculated (or injected) with human renal cell carcinoma, prostate cancer and DU-145 cell lines by subcutaneous, in situ and subrenal transplantation. Rate, liver disease (clinical manifestations, pathology, distribution of death time) and so on. Results: The total tumorigenic rate of transplanted renal cell carcinoma was 21.7% (35/161) and the metastasis rate was 1.2% (2/161). The transplanted DU-145 cell lines had a tumorigenic rate of 100% (20/20) and a metastatic rate of 25% (5/20). The rate of hepatic lesion was 58.4% (94/161) in transplanted renal cell carcinoma and 43.4% (46/106) in transplanted prostate cancer, but none in DU-145 cell line Liver disease occurs. Transplanted renal cell carcinoma, prostate cancer specimens of liver lesions in mice peak mortality and peak mortality trends are consistent, occur in the annual winter and spring. Conclusion: Liver disease is the main cause of abnormal animal death. Strict control of SPF environment can ensure the establishment of tumor animal model.