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本文用枯草杆菌重组修复试验(芽孢法)和大肠杆菌回变试验方法,研究了饮水消毒剂一氯氨(NH_2Cl)的致突变性。结果显示:NH_2Cl在检测DNA损伤及修复的枯草杆菌重组修复试验中,在加和不加S_9的条件下,当浓度分别为:3.24、6.47、12.94mM和3.64、7.28、14.56mM时,均未检出致突变性;在检测基因突变的大肠杆菌回变试验中,在加和不加S_9的条件下,当浓度分别是:2.82×10~(-3)、1.41×10~(-2)、7.04×10~(-2)mM和2.74×10~(-3)、1.37×10~(-2)、6.84×10~(-2)mM时,均获得了阳性结果。根据国际预访环境致突变物致癌物委员会意见,有理由认为,在本实验条件下,NH_2Cl为一致突变剂。
In this paper, Bacillus subtilis recombinant test (spore method) and Escherichia coli back-change test method to study the water disinfectant, monochloroammine (NH_2Cl) mutagenicity. The results showed that when NH_2Cl was used to detect the DNA damage and repair in Bacillus subtilis, the results showed that when NH_3Cl was added and without S_9, the concentrations were 3.24,6.47,12.94mM and 3.64,7.28,14.56mM respectively, In the test of E.coli mutagenesis, the results showed that when the concentration of S_9 was 2.82 × 10 -3 and 1.41 × 10 -2, , 7.04 × 10 ~ (-2) mM and 2.74 × 10 ~ (-3), 1.37 × 10 ~ (-2) and 6.84 × 10 ~ (-2) mM, respectively. According to the opinion of the International Commission on Cancer Mutation Mutagenicity on Pre-Environmental Surveillance, it is reasonable to assume that NH2Cl is a consistent mutant under the conditions of this experiment.