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研究了日本小麦品种与四倍体球茎大麦的可杂交性以及由胚培养产生单倍体。采用高湿以及授粉后施用赤霉素的杂交程序,提高了种子结实率,且小花成熟良好。在8个供试的日本小麦品种中,有7个品种可与球茎大麦杂交,平均种子结实率为4.4～24.0％。通过胚培养,半数左右的日本小麦品种×球茎大麦的杂种胚发育成单倍体小麦植株。本结果表明,与球茎大麦杂交的双单倍体方法有希望应用于日本的小麦育种计划。
The crossability of Japanese wheat cultivars with tetraploid bulblets and the haploid generation from embryo culture were studied. The hybridization procedure with high humidity and application of gibberellin after pollination improved the seed setting rate and the floret mature well. Among the eight Japanese wheat cultivars tested, seven cultivars were crossed with bulblet barley with an average seed set rate of 4.4-24.0%. Through embryo culture, about half of hybrid embryos of Japanese wheat × bulblets developed into haploid wheat plants. This result shows that the double haploid method of crossing with bulblet barley has the hope of being applied to the Japanese wheat breeding program.