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目的:探讨慢性淋巴细胞性甲状腺炎(CLT)患者外周血辅助性T细胞17(Th17)的水平及意义。方法:收集甲状腺功能正常组CLT患者15例,甲状腺功能减退组CLT患者30例及20例健康对照组研究者的外周血样。采用胞内细胞因子染色流式细胞术(FCM)分析法,检测各组外周血Th17细胞占CD4+T细胞比值。应用电化学发光法检测CLT患者及健康对照组血清中抗甲状腺过氧化物酶抗体(TPO-Ab)和抗甲状球蛋白抗体(TG-Ab)水平。结果:与健康对照组比较:甲状腺功能正常组及甲状腺功能减退组的CLT患者外周血Th17/CD4+T细胞比值分别为(3.64±1.96)%、(3.13±1.56)%,均高于健康对照组(0.50±0.32%,P<0.01);两组CLT患者血清TPO-Ab和TG-Ab水平分别为(398.99±222.88;1456.02±1054.97)IU/L和(423.26±167.21;1587.94±1210.36)IU/L,均明显高于健康对照组(15.23±10.36;36.32±26.99)IU/L;差异有统计学意义(P<0.01)。CLT患者外周血Th17/CD4+T细胞比值改变与血清TPO-Ab及TG-Ab阳性表达呈显著正相关(分别为r=0.50,r=0.43;P<0.01)。结论:两组CLT患者外周血Th17细胞比率增加,且与甲状腺自身抗体水平存在显著正相关,Th17细胞可能参与CLT的发生发展。
Objective: To investigate the level and significance of peripheral blood helper T cells (Th17) in patients with chronic lymphocytic thyroiditis (CLT). Methods: Peripheral blood samples were collected from 15 CLT patients with normal thyroid function, 30 CLT patients with hypothyroidism and 20 healthy controls. Using intracellular cytokine staining flow cytometry (FCM) analysis, the detection of peripheral blood Th17 cells accounted for CD4 + T cell ratio. The levels of anti-thyroid peroxidase antibody (TPO-Ab) and anti-thyroglobulin antibody (TG-Ab) in serum of patients with CLT and healthy controls were detected by electrochemiluminescence. Results: Compared with healthy control group, the Th17 / CD4 + T cell ratio in peripheral blood of normal thyroid function group and hypothyroidism group were (3.64 ± 1.96)% and (3.13 ± 1.56)%, respectively, which were higher than that of healthy control (0.50 ± 0.32%, P <0.01). The levels of serum TPO-Ab and TG-Ab in two groups of patients with CLT were (398.99 ± 222.88; 1456.02 ± 1054.97) IU / L and (423.26 ± 167.21; 1587.94 ± 1210.36) IU / L, were significantly higher than the healthy control group (15.23 ± 10.36; 36.32 ± 26.99) IU / L; the difference was statistically significant (P <0.01). The Th17 / CD4 + T cell ratio in peripheral blood of patients with CLT was positively correlated with the positive expression of TPO-Ab and TG-Ab (r = 0.50, r = 0.43, P <0.01). Conclusion: Th17 cells in peripheral blood of two groups of patients with CLT increase in proportion, and the level of thyroid autoantibodies there is a significant positive correlation, Th17 cells may participate in the occurrence and development of CLT.