目的探讨苍术苷(ATR)抑制氧化磷酸化电子传递链可能的作用靶点。方法将制备好的线粒体悬液分为对照组、N-乙酰-L-半胱氨酸(NAC)组、鱼藤酮(ROT)组、NAC和ROT联合组,每组再分为4小组,分别用0,20,40,100滋mol·L-1的ATR处理。测定各组线粒体呼吸链中4种蛋白质复合体和ATP酶的活性。结果 ATR能够抑制复合体Ⅰ、Ⅳ和2种ATP酶的活性,且其抑制作用和剂量相关。结论 ATR可能影响呼吸链电子传递的始端复合体Ⅰ、末端状态复合体Ⅳ和ATP酶活性,最终抑制ATP的形成。 Objective To investigate the possible targets of atractyloside (ATR) in inhibiting the oxidative phosphorylation of electron transport chains. Methods The mitochondrial suspension was divided into control group, NAC group, rotenone group (ROT), NAC group and ROT group. Each group was divided into 4 groups, 0, 20, 40, 100 mol L-1 ATR treatment. The activities of four protein complexes and ATPase in mitochondrial respiratory chain of each group were determined. Results ATR inhibited the activity of complex Ⅰ, Ⅳ and 2 ATPase, and its inhibitory effect was dose-dependent. Conclusion ATR may affect the initiation of terminal respiratory chain electron transport complex Ⅰ, the terminal state of the complex Ⅳ and ATPase activity, and ultimately inhibit the formation of ATP.