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应用ＳＰ法（链霉菌抗生物素蛋白─过氧化酶连结法）对肝细胞癌癌组织（４６例）及其癌旁组织（３８例）中丙型肝炎病毒ＮＳ３抗原的免疫组织化学染色发现，两者的检出率分别为２３．９％和３９．５％。ＮＳ３抗原阳性染色细胞呈弥漫、灶状和散在分布，阳性染色信号呈细颗粒状，定位于肝细胞和肝癌细胞的胞浆内。抗原表达与局部的病理形态改变之间无明显的联系。结果提示ＮＳ３抗原是丙型肝炎病毒感染后的表达蛋白，而且ＨＣＶ感染在肝细胞癌的发生中可能起重要作用。ＨＣＶＮＳ３抗原的检测可作为ＨＣＶ感染的另一标志物。ＨＣＶＮＳ３抗原在肝细胞癌发生中的真正作用，尚待进一步研究。
Immunohistochemical staining of hepatitis C virus NS3 antigen in hepatocellular carcinoma tissues (46 cases) and paracancerous tissues (38 cases) using SP method (streptavidin-peroxidase linkage method) The detection rates of the two were 23.9% and 39.5% respectively. The positive staining cells of NS3 antigen showed diffuse, focal and scattered distributions. The positive staining signal was fine-grained and located in the cytoplasm of hepatocytes and liver cancer cells. There is no clear correlation between antigen expression and local pathological changes. The results suggest that NS3 antigen is an expressed protein after hepatitis C virus infection, and HCV infection may play an important role in the occurrence of hepatocellular carcinoma. Detection of the HCV NS3 antigen may serve as another marker of HCV infection. The true role of HCV NS3 antigen in the development of hepatocellular carcinoma remains to be further studied.