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[目的]用体外实验方法研究1,2-二氯乙烷(1,2-DCE)染毒后大鼠神经细胞内钙离子(Ca2+)浓度的变化。[方法]体外培养新生SD大乳鼠脑皮质细胞,分别用0.5、1.0、2.0mL/L 1,2-DCE染毒,另设对照组,观察各组神经细胞形态学变化。同时应用激光共聚焦显微镜测定各组细胞内Ca2+浓度,探讨1,2-DCE对大鼠神经细胞内Ca2+浓度的影响。[结果]神经细胞染毒后,神经细胞形态发生明显改变:胞体肿胀崩解、胞核模糊不清、突触变短变粗、细胞间连接减少、细胞膜不完整;随着染毒剂量的增高,神经细胞损伤的严重程度呈上升趋势。各染毒组神经细胞活力较对照组有所下降,差异有统计学意义(P<0.01);染毒2h后随着染毒剂量的增加,各组神经细胞内Ca2+浓度呈上升趋势,各染毒组细胞与对照组比较差异有统计学意义(P<0.01);染毒24h后,低、中剂量组与对照组细胞内Ca2+浓度比较差异有统计学意义(P<0.01),高剂量组与对照组比较差异无统计学意义(P>0.05)。[结论]1,2-DCE可以导致神经细胞水肿坏死,有剂量依赖关系,神经细胞内Ca2+浓度亦见升高,提示Ca2+可能与1,2-DCE致神经细胞中毒性脑水肿有关。
[Objective] To study the changes of intracellular calcium (Ca2 +) concentration in neurons after 1,2-DCE exposure in vitro. [Methods] The cerebral cortical neurons of newborn SD neonatal rats were cultured in vitro. The cells were treated with 0.5, 1.0 and 2.0 mL / L 1, 2-DCE respectively. The control group was also established to observe the morphological changes of the neurons. At the same time, the intracellular Ca2 + concentrations in each group were determined by laser confocal microscopy, and the effect of 1,2-DCE on the intracellular Ca2 + concentration in neurons was investigated. [Results] The morphological changes of nerve cells were significantly changed after the neurons were exposed to the virus. The swelling and swelling of the cell body, the unclear nucleus, the short and thick synapses, the decrease of the intercellular connection and the incomplete cell membrane were observed. , The severity of nerve cell injury is on the rise. Compared with the control group, the activity of nerve cells in each exposure group decreased significantly (P <0.01). After exposure for 2h, the intracellular Ca2 + concentration in neurons increased with the increase of exposure dose, Compared with the control group, there was significant difference between the poisoned group and the control group (P <0.01). The intracellular Ca2 + concentration in the low and middle dose groups was significantly different from that in the control group (P <0.01) Compared with the control group, there was no significant difference (P> 0.05). [Conclusion] 1,2-DCE can cause neuronal edema and necrosis in a dose-dependent manner, and the intracellular Ca2 + concentration also increases, suggesting that Ca2 + may be related to toxic brain edema induced by 1,2-DCE in neurons.