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目的建立高效液相色谱法(HPLC)同时测定妇炎消胶囊中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量。方法采用Diamonsil(TM)C18分析柱(250 mm×4.6 mm,5μm),流动相为甲醇∶0.1%磷酸溶液(85∶15),流速为1.0 ml/min,柱温为25℃,检测波长为254 nm。结果芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚线性范围分别为0.04～0.40μg、0.09～0.90μg、0.09～0.90μg、0.13～1.30μg、0.174～1.740μg;平均加样回收率(n=5)为97.41%(RSD=2.94%)、100.58%(RSD=0.44%)、98.47%(RSD=1.28%)、97.60%(RSD=1.53%)、96.76%(RSD=3.20%)。结论该方法简便快速,结果准确可靠,能较好地控制产品质量。
OBJECTIVE To establish a HPLC method for simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Fuyanxiao capsule. Methods Diamonsil TM C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of methanol: 0.1% phosphoric acid solution (85:15), the flow rate was 1.0 ml / min and the column temperature was 25 ℃ 254 nm. Results The linear ranges of aloe-emodin, rhein, emodin, chrysophanol and emodin were 0.04 ~ 0.40μg, 0.09 ~ 0.90μg, 0.09 ~ 0.90μg, 0.13 ~ 1.30μg and 0.174 ~ 1.740μg, respectively. The recoveries (n = 5) were 97.41% (RSD = 2.94%), 100.58% (RSD = 0.44%), 98.47% (RSD = 1.28%), 97.60% (RSD = 1.53%), 96.76% %). Conclusion The method is simple and rapid, the result is accurate and reliable, and the product quality can be well controlled.