用人体肺腺癌细胞(LAX)脾内一次性免疫BALB/C小鼠后,将脾细胞与小鼠骨髓瘤细胞(NSO)融合,融合率为80％,对LAX细胞反应阳性率为20％。以酶联免疫吸附试验,免疫荧光试验和免疫组织化学试验,检测其与正常人体细胞,人体肿瘤细胞和人体肺腺癌的反应,发现获得的ALA-05单克隆抗体与正常人体细胞无交叉反应,与多株人体其他肿瘤细胞也无反应,而与人体肺腺癌则呈阳性反应,且为抗膜抗体。免疫扩散法证明其是IgG_(2a)。 BALB / C mice were immunized once with the spleen of human lung adenocarcinoma cells (LAX). The spleen cells were fused with mouse myeloma cells (NSO) at a confluent rate of 80% and a positive rate of 20% on LAX cells . The results of enzyme-linked immunosorbent assay (FACS), immunofluorescence assay and immunohistochemistry assay showed that the monoclonal antibody against ALA-05 had no cross reaction with normal human somatic cells, human tumor cells and human lung adenocarcinoma. , And many other human tumor cells did not respond, but with human lung adenocarcinoma was a positive reaction, and anti-membrane antibody. Immunodiffusion proved it to be IgG_ (2a).