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目的探讨脱氢表雄酮(DHEA)对小鼠成骨细胞(OB)增殖周期与凋亡的调控作用。方法颅骨酶解法分离OB和体外培养OB,体外模拟雌激素撤退现象,分为对照组、10~(-10)mol/L E_2组、10~(-7)mol/L DHEA组共3组,流式细胞仪分析DNA含量,Annexin V-FITC/PI双标记流式细胞仪分析细胞早期凋亡,透射电镜观察OB超微结构。结果DNA含量分析显示,DHEA组的S期和G_2/M期细胞百分率比对照组明显增加,细胞增殖指数显著增加(P＜0.01)；DHEA和E_2对OB还具有显著的抗凋亡作用(P＜0.01或P＜0.05)；透射电镜可见经10~(-7)mol/L DHEA诱导后的OB细胞器明显增多,内质网扩张,线粒体丰富等形态学改变。结论DHEA在体外可直接促进小鼠OB的增殖并抑制其凋亡。
Objective To investigate the regulatory effect of dehydroepiandrosterone (DHEA) on the proliferation and apoptosis of mouse osteoblasts (OB). Methods OB and in vitro cultured OB were cultured by skull enzymolysis. The estrogen withdrawal was simulated in vitro and divided into control group, 10 ~ (-10) mol / L E2 group, 10 ~ (-7) mol / DNA content was analyzed by flow cytometry. Cell apoptosis was analyzed by Annexin V-FITC / PI double-labeled flow cytometry. The ultrastructure of OB was observed by transmission electron microscope. Results DNA content analysis showed that the percentage of cells in S phase and G 2 / M phase in DHEA group was significantly higher than that in control group and the cell proliferation index was significantly increased (P <0.01). DHEA and E 2 had significant anti-apoptotic effect on OB (P <0.01 or P <0.05). Transmission electron microscopy showed that the number of OB organelles induced by 10 -7 mol / L DHEA increased significantly, and the changes of endoplasmic reticulum, mitochondria and other morphological changes. Conclusion DHEA can directly promote the proliferation and inhibit the apoptosis of OB in mice.