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以‘台农1号’杧果为试材,用1–甲基环丙烯(1-MCP,1μL·L-1)常温下处理12 h和未处理的果实为对照,通过数字基因表达谱(DGE)技术,寻找1-MCP处理对杧果贮藏影响的潜在关键基因。DGE数据分析结果表明,1-MCP处理与对照样品文库相比,共检测到7 350个差异表达基因。其功能主要涉及细胞壁代谢,激素调节,逆境胁迫应答,氧化损伤保护,能量代谢,蛋白质折叠,泛素化和蛋白酶体途径介导的细胞程序性死亡,成熟与衰老调控等。利用实时定量PCR(q RT-PCR)技术对DGE部分数据进行验证,检测了其中6个较有代表性的应答基因的差异表达。通过基因本体(Gene Ontology,GO)通路功能富集分析表明,1-MCP处理增加果实对逆境胁迫的抵抗能力,抑制物质和能量代谢,减少细胞内钙的流失并保护果实细胞。qRT-PCR技术数据支持RNA-Seq的检测结果。
The fruit of ’TINONG 1’ was used as control, and treated with 1-methylcyclopropene (1-MCP, 1 μL·L-1) at room temperature for 12 h and untreated fruits as control. DGE) technology to find the potential key genes affecting the storage of 1-MCP. DGE data analysis showed that 7 350 differentially expressed genes were detected in 1-MCP-treated and control samples. Its function mainly involves cell wall metabolism, hormone regulation, stress response, oxidative damage protection, energy metabolism, protein folding, ubiquitination and proteasome pathway-mediated apoptosis, maturation and aging regulation. The real-time quantitative PCR (q RT-PCR) was used to validate the data of DGE, and the differential expression of six representative response genes was detected. Gene enrichment analysis of Gene Ontology (GO) pathway showed that 1-MCP treatment could increase the resistance of fruit to stress, inhibit the metabolism of matter and energy, reduce the intracellular calcium loss and protect the fruit cells. qRT-PCR data support RNA-Seq test results.