论文部分内容阅读
目的:为开发利用金樱根多糖及制定其质量标准提供依据。方法:采用超声波强化碱水水浴提取法,通过单因素和正交实验对提取工艺进行优选,考察氢氧化钠浓度、料液比、提取温度、提取时间对多糖得率的影响,多糖含量测定采用硫酸-苯酚法。结果:优选的提取工艺为氢氧化钠浓度2.5%,料液比1∶90,提取温度100℃,提取时间2.5h。多糖含量测定得标准曲线回归方程为Y=7.784 2X+0.000 9,r=0.999 3。结果表明,在溶液浓度为0～0.05mg/mL范围内测定葡萄糖吸光度呈良好线性。平均回收率为94.70%,RSD=2.51%(n=6)。各因素对金樱根多糖得率的影响由大到小依次为:料液比>提取时间>氢氧化钠浓度。多糖得率(124.593 8±6.150 6)mg/g。结论:采用超声波强化碱水水浴提取法,多糖提取完全,提取工艺设计合理,稳定可行,操作简便。
Objective: To provide the basis for the development and utilization of the gold sap root polysaccharide and to develop its quality standards. Methods: The extraction process was optimized by single factor and orthogonal experiment with ultrasonic enhanced alkaline water bath extraction method. The effects of sodium hydroxide concentration, solid-liquid ratio, extraction temperature and extraction time on polysaccharide yield were investigated. The polysaccharide content was determined by Sulfuric acid - phenol method. Results: The optimal extraction process was sodium hydroxide concentration 2.5%, solid-liquid ratio 1:90, extraction temperature 100 ℃ and extraction time 2.5 h. The standard curve regression equation of polysaccharide content was Y = 7.784 2X + 0.000 9, r = 0.999 3. The results showed that the absorbance of glucose was linear with the concentration of 0 ~ 0.05mg / mL. The average recovery was 94.70%, RSD = 2.51% (n = 6). The effects of various factors on the yield of polysaccharides were descending order: solid-liquid ratio> extraction time> sodium hydroxide concentration. Polysaccharide yield (124.593 8 ± 6.150 6) mg / g. Conclusion: Ultrasonic-enhanced alkaline water bath extraction method, extraction of polysaccharides completely, the extraction process design is reasonable, stable and feasible, easy to operate.