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目的测定养血安神糖浆中5-羟甲基糠醛的含量。方法采用HPLC法测定5-羟甲基糠醛的含量。色谱柱:SHISEIDO CAPCELL PAK C18柱,250mm×4.60mm,5μm;流动相:甲醇-0.1mol/L磷酸溶液(10 90);检测波长:284nm;柱温:45℃;流速:0.8ml/min;进样量:10μl。结果 y=899702.71x+26090.44,R=0.9997,线性范围为0.40μg/ml-19.70μg/ml,平均回收率为98.3%。结论方法简便,快速,准确,为提高和完善该制剂的质量标准提供了依据。
Objective To determine the content of 5-hydroxymethylfurfural in nourishing and tranquilizing syrup. Methods The content of 5-hydroxymethylfurfural was determined by HPLC. Column: SHISEIDO CAPCELL PAK C18 column, 250 mm × 4.60 mm, 5 μm; mobile phase: methanol -0.1 mol / L phosphoric acid solution (10 90); detection wavelength: 284 nm; Injection volume: 10μl. The results y = 899702.71x +26090.44, R = 0.9997, the linear range of 0.40μg / ml-19.70μg / ml, the average recovery was 98.3%. Conclusion The method is simple, rapid and accurate, which provides a basis for improving and perfecting the quality standard of the preparation.