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对杨属青杨派主要树种大青杨、甜杨、香杨和马氏杨种间及种内遗传变异进行了RAPD检测,结果表明:7个随机引物对4个树种DNA扩增产物绝大部分呈现为单型性,而有3个引物扩增产物显示出丰富的种间多型性;所有引物均在4个树种内扩增出不同程度的多态性。统计分析建立的系统树还说明了4树种可能的亲缘关系及系统发育史,同时,各树种内也均存在着遗传多样性。这对今后进行这些树种的遗传改良具有重要意义。此外还得出:分子水平分类的结果与经典分类一致:4个树种中马氏杨出现最早,甜杨次之,香杨再次之,大青杨最晚;大青杨与香杨亲缘关系最近,因此,以往认为大青杨与甜杨亲缘关系最近的说法有必要重新审视。
The results of RAPD analysis of interspecific and intraspecific genetic variations among poplar, Populus tomentosa, Populus tomentosa and Populus simonii, the main tree species of Populus simonii, showed that the random amplified polymorphic DNA Part of them showed monomorphism, while three primers showed abundant polymorphism. All the primers amplified polymorphisms in four species. The phylogenetic tree established by statistical analysis also shows the possible phylogenetic relationships and phylogenetic relationships among the 4 species, as well as the genetic diversity within each species. This is of great significance for future genetic improvement of these species. In addition, the results of molecular classification are the same as those of the classical classification. Among the four tree species, the male poplar appeared first, the sweet poplar took second place, the fragrant poplar again, the young poplar the latest, the closest relationship between Populus euphratica and Fragrant poplar Therefore, in the past that Poplar and Sweet Yang kinship recent claims need to re-examine.