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利用巯基丙酸包覆的In P@Zn S量子点(QDs)与Dured构建了一种检测DNA的荧光探针。在该探针中,以环境友好型带负电的In P@Zn S量子点为荧光团,与带正电的Dured通过静电结合,构建了In P@Zn S QDs/Dured纳米荧光探针。通过荧光共振能量转移(FRET)机理,量子点荧光被猝灭;当DNA存在时,Dured与DNA的特异性结合使Dured从In P@Zn S QDs表面脱附,FRET过程被打断,In P@Zn S QDs荧光恢复,以荧光“关-开”方式检测DNA。该探针检测DNA的线性范围为2.0~275.0 ng·L-1,检测限为1.0 ng·L-1,并可用于模拟生物生理条件下的DNA检测。
A fluorescent probe for detecting DNA was constructed using InP @ ZnS quantum dots (QDs) coated with mercaptopropionic acid and Dured. In this probe, an In P @ Zn S QDs / Dyed nanofluorescent probe was constructed by electrochemically binding an environmentally friendly negatively charged In P @ ZnS quantum dot as a fluorophore to a positively charged Dured. The quantum dot fluorescence is quenched by the fluorescence resonance energy transfer (FRET) mechanism. When DNA is present, the specific binding of Dured to DNA causes the desorption of Dured from the In P @ Zn S QDs surface, the FRET process is interrupted and In P @Zn S QDs fluorescence recovery, fluorescence “off - on ” way to detect DNA. The linear range of this probe for DNA detection was 2.0-275.0 ng · L-1 with a detection limit of 1.0 ng · L-1 and could be used to simulate DNA detection under physiological conditions.